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Prof.
Dr. Feride Severcan
Professor of
Biophysics
Dept. of Biological
Sciences
Middle East Technical
University
06531 Ankara
Turkey
www.metu.edu.tr
Tel: 90-312-2105166
(office); 90-312-2103103 (secretary)
90-533-5768975 (cellular)
Fax: 90-312-2101289
feride@metu.edu.tr
GENERAL
INFORMATION ABOUT MIDDLE EAST TECHNICAL UNIVERSITY (METU)
The Middle East Technical University is devoted to the pursuit and
application of knowledge for the social, cultural, economic, scientific and
technological development of our society and mankind through achievements in
teaching, research and community service that are of highest international
standards. The University strives to be a university of the first rank in an
international context. To this end, the University seeks to be a dynamic
institution aiming at excellence that will attract both the best teaching and
research staff and the highest quality students.
METU is one of Turkey's most competitive
universities. Each year, among the students taking the National University
Entrance Examination, over 1/3 of the 1000 applicants with the highest scores
attend METU. Due to the high demand towards METU is so great, most of our
departments accept only the top 1% of the approximately 1.5 million
applicants taking the National University Entrance Examination. Over 40%
of METU's students go on to graduate school.Nearly 19,000 students are
registered to METU in an academic year. Approximately 25% of these are enrolled
in graduate programs. Each year, between 800 to 1200 students with various
academic degrees from nearly 50 different foreign countries attend METU.
Undergraduate and graduate students from a number of countries attend METU as
"Special Students" or "Exchange Students" for a semester or
a year. METU currently has active Student Exchange Programs with several foreign
universities.
METU has 37 undergraduate programs
within 5 faculties. Additionally, there are 5 Graduate Schools with 78
programs and a "School of Foreign Languages" which includes the
English Preparatory Department.
METU strives to maintain a high standard of education by international
standards. Several departments in the Engineering Faculty have been evaluated,
at METU's request, by the "Accreditation Board for Engineering and
Technology (ABET)" and their programs were given approval by this
Board. Because of METU's effort to maintain international standards, the
"Faculty of Engineering" was awarded in 1977 the "Silver Badge
of Honor" by the "UNESCO International Center for Engineering
Education" and the "Meritorious Achievement Award in Accreditation
Activities" by the "American Institute of Electrical and
Electronic Engineers".
The campus area is 4500 hectares and the forest area is 3043
hectares, including Lake Eymir, about 20 kilometers from the centrum
of Ankara. The lake is available to METU students for rowing, fishing,
picnicking and general recreational activities. Bus service is provided from the
METU main campus and locations in the city to the lake.
PROPOSAL
Molecular
mechanism of system disordes induced by metabolic diseases: Effect of several
drugs on the treatment of organ disorders.
Prof.
Dr. Feride Severcan (Project Coordinator)
Dept.
of Biological Sciences, Middle East Technical University, 06531, Ankara-Turkey
Tel:
90-312-2105166; Fax: 90-312-2101289; E-Mail: feride@metu.edu.tr
Web
address of METU: www.metu.edu.tr
Summary:
The
effect of diabetes mellitus on the content, structure and function of cell
components such as lipids, proteins
and nucleic acids, in body fluids
obtained from normal and diabetic patients and on different organs of diabetic
animal models will be investigated at molecular level by Fourier transform
infrared ( FTIR) spectroscopy inconjuction with FTIR microscopy and
differential scanning calorimetry (DSC). In addition to this, biochemical
blood parameters including antioxidant enzyme levels will be determined. The
effects of experimental diabetes on elevated glucose and free radicalson the
NO-mediated relaxation of isolated the esophageal smooth muscle (tunica
muscularis mucosae), lower esophageal sphincter, ileum and duodenum smooth
muscle strips will be studies using
in vitro muscle technique.Effect of genotypes on the
development of diabetes will be examined. Electrophysiological studies will also
be performed to better understand the effect of diabetes on cardiovascular
disorders. Neural networks will be used to improve data analysis in FTIR
spectroscopy and to
classify FTIR spectra and electrophysiological signals for the diagnosis of
diabetes. Furthermore,
the effect of several drugs such as different antioxidants on body fluids,
erythrocyte membranes in human patients and different organ disorders in
radiated and unirradiated animal models will be investigated.
Introduction:
It
has been known that diabetic patients have a tendency towards cardiovascular
system disorders, including congestive heart insufficiency, hypertension,
atherosclerosis, micro- and macro- angiopathy and together with these, various
smooth muscle functions have been negatively affected by this disease. Moreover,
it has been demonstrated that, there are mortalities due to diabetic
cardiomyopathy. Although functional disorders of heart have been determined both
clinically and experimentally, the mechanisms behind these pathologies have not
been completely understood yet. It is known that, diabetes mellitus leads to the
changes in fluidity and molecular dynamics of the membranes of sarcolemma and
other organelles, and to the inhibition of several ionic channels, pumps and to
the exchange mechanisms, which are present in membranes as well as alterations
in cardiac electrophysiology. In recent years, the effect of diabetes on
membranes of blood cells, like erythrocytes, leukocytes and platelets, have been
studied extensively. However, the effects of this disease on the molecular
structure and dynamics, and also function of heart and liver tissue homogenates
and cell membranes have not been investigated in detail yet. A kind of myopathy
in heart of diabetic people and also in animal models is defined which is called
diabetic cardiomyopathy. Furthermore, osteoporosis is one of the most important
complications of diabetes, especially insulin dependent diabetes mellitus (type
I). Diabetes mellitus can affect bone formation and resorption by creating
several metabolic irregularities in acid-base, calcium-phosphate balances.
However, in diabetic bone tissues; the changes in the concentration,
distribution and type of carbonate and phosphate groups and the effects of these
changes on the crystallinity of bone mineral structure have not been reported
yet. In most diabetic patients, the motor functioning of the esophagus is
impaired. Esophageal dismotility is found in the majority of diabetic patients
with periferal or autonomic neuropathy. As in all of the gastrointestinal
system, the extrinsic control of the motility of esophagus and lower esophageal
sphincter (LES) is maintained by the sympathetic and the parasymphatetic nervous
system; and its intrinsic control is maintained by the enteric nervous system.
Intrinsic nervous system plays a major role in the ethiopathogenesis of GIS
motility disorders induced by diabetes mellitus.
Free radicals are generated in biological systems as a result of
exposure to a wide variety of external factors including some drugs, pollutants,
heavy metals, heat, UV or visible light, and other forms of ionizing radiation.
The generation of these reactive species in an uncontrolled fashion causes
significant reversible or irreversible damage to a wide range of biological
molecules including DNA, proteins, carbohydrates, and lipids. Many of these
processes are chain reactions with a single initiating radical species which is
propagated to a large number of target molecules. One of their main target is
biological membranes by causing lipid peroxidation which ends up with the damage
of cells. So there is a considerable interest in the reactions of these species,
the damage they induce on membranes and their relationship in the physiology and
pathology of a variety of diseases and processes including cancer, arthritis,
ageing and heart disease. In combatting the harmful effects of free radical
tissue damage, it is vital that antioxidant defense mechanisms are localized at
the sites of potential damage and that they are primed to maximum effectiveness.
Vitamin E is minor but ubiquitous component present among the lipid constituents
of cell membranes and lipoproteins. Vitamin C is a soluble vitamin but both
vitamins prevent the oxidation of cellular constituents acting as antioxidants.
GSH and GSH-associated enzymes such as glutathione peroxidase, GSTs, superoxide
dismutase and catalase are the major enzymes which protect the cell against free
radical damage. Though the individual protective roles of those systems were
known, there was no study in literature emphasizing on the collective roles of
those systems.
Description of the project
In
the first part of the present study, diabetes will be induced in rats by
intraperitonial application of streptozotosin, which impairs the function of
pancreatic beta cells, thereby prevents the secretion of insulin. To investigate
diabetic cardiomyopathy under STZ-induced diabetes, different
electrophysiological techniques will be used. We will use Langendorrff system
for monitoring heart contractile force, heart rate and its derivatives.
Intracellular microelectrode technique will measure membrane potential changes
and action potential from small heart strips. Ionic channel currents such as Ca,
K, will be measured by patch-clamp technique. For Intracellular pH and Ca
measurements microflourometric technique will be used. For molecular studies,
the soft tissues like liver and heart; and the hard tissues like bone (tibia and
femur) will be investigated. For this purpose, FTIR
spectroscopy and FTIR
microscopy will be used to obtain detailed information about the effects of
diabetes on different biological systems. FTIR spectroscopy is an excellent
technique to monitor, simultaneously, the structural, functional and
compositional changes in specific functional groups belonging to lipids,
proteins, polysaccarides, and nucleic acids in biological systems. FTIR
microscopy, which has been extensively used in recent years for imaging, reveals
the detailed qualitative and quantitative analysis of soft and hard tissues in a
short time by using very small amounts of samples. In the present work, FTIR
microscopy will provide us to monitor the pathological regions induced by
diabetes in different section of the tissues. The results of diabetic tissues
and membranes will be compared with the results of nondiabetic ones to
understand the effect of diabetes at molecular level. The effect of diabetes on
antioxidant enzymes will be determined. All these experiments will be performed
for normal and irradiated diabetic samples.
In
the content of molecular studies, population genetic studies will also be
performed: Glyoxylase
I (Glo I), which catalyses the conversion of methylglyoxal to S-D lactoyl
glutathione is a dimeric enzyme having two alleles. A human can be in one of the
three possible genotypes (GloI 11, GloI 12, GloI 22) with respect to Glo I
locus. It has been shown that GloI 22 genotype has a significantly higher
frequency in insulin independent (type
II) diabetic patients compared to control. In the proposed study association of
GloI genotypes with diabetics (type I and II) in Turkish population will be
confirmed and the risk of diabetes for GloI
genotypes will be determined. Futhermore, blood and saliva samples of
three genotypes in typeI, typeII diabetics and
normal group will be examined comparatively by FTIR spectroscopy. Ease in
the induction of diabetes in lab animals, their GloI based genotypes and their
FTIR spectra will also be studied. The study will give information about the
mechanism of diabetes.
The
effects of experimental diabetes on the nicotine–induced relaxation of the
esophageal smooth muscle (tunica muscularis mucosae), lower esophageal
sphincter, ileum and duodenum smooth muscle will be investigated. In addition,
the effect of glucose on nicotine-induced relaxant responses will be determined
by incubating isolated rat ileum, duodenum, tunica muscularis mucosae and lower
esophageal sphincter smooth muscle in control (11 mM) or elevated (44 mM)
glucose for 6 h to mimic euglycemic and hyperglycemic conditions. The
role of free radicals on the changed responses of gastrointestinal smooth
muscles will be examined with free radical scavengers.
Since
antioxidants have a protective role in oxidative stress induced diseases, in the
second part of the project, the effect of different antioxidants as drugs on the
treatment of the damages induced by diabetes mellitus will be investigated.
Neural
networks will be used to improve data analysis in FTIR spectroscopy and to
classify FTIR spectra and electrophysiological signals for the diagnosis of
diabetes.
Group Members
Prof.
Dr. Feride Severcan: METU- Biophysical Studies
Prof.
Dr. İnci Togan: METU- Population Genetic and Statisctical Studies
Prof.
Dr. Tülin Güray: METU-Biochemical Studies
Prof.Dr.Mete
Severcan: METU-Bioinformatics
Prof.Dr.
Belma Turan: Ankara University-Electrophysiological Studies
Prof.Dr.
Faruk Zorlu: Hacettepe University-Radiation Oncology
Prof.Dr.
Sevgi Bayari: Hacettepe University-Biophysical Studies
Prof.
Dr. Nadide Kazancı: Ege University-Biophysical Studies
Assist.
Prof. Senay Baysal: Ege University-Biochemical Studies
Prof.
Dr. Tijen Utkan: Kocaeli University-Pharmacalogical Studies
Assist.
Prof. Kıvanc Ergen: Kocaeli University-Biophysical Studies
Assist
Prof: Firuzan Yıldız: Kocaeli University-Pharmacological Studies
Dr.
Kübra Paskaloğlu-Marmara University-Pharmacological Studies
Handan
Boyar (M.Sc.)- METU- Biophysical Studies
Neslihan
Toyran Al-Otaibi (M.Sc, Research Assistant)-METU-Biophysical Studies
Güvenç
Görgülü (M.Sc, Teaching Assistant)-METU-Biochemical and Biophysical Studies
Ayça
Doğan (M.Sc-Lecturer)-Kocaeli University-Biophysical Studies
Gülgün
Çakmak (M.Sc)-METU-Biophysical Studies
Sevgi
Türker(M.Sc-Teaching Assistant)-METU-Biophysical Studies
Evren
Koban (M.Sc-Research Assistant)- METU- Population Genetic Studies
And
several B.S and M.S students
SHORT
CV s of GROUP MEMBERS
Feride
SEVERCAN (Prof. Dr,) : Gender: Female, Dept.of Biological Sciences, METU. E-mail:
feride@metu.edu.tr.
B.S.
in Ankara University, Turkey, M.S.
in Rochester Univ., U.S.A.;
Ph.D. in Hacettepe Univ., Ankara, Turkey. Post Doctoral study in Stanford University and San Francisco State
University. Lecturer in San Francisco State University. Research Associate in
Perugia University, Italy. Visiting Scientists in U.C. Santa Cruz, USA,
Bath Univ: U.K., De Montford Univ., U.K
BATTELLE, USA. Research Interests:
Molecular investigation of normal and pathological tissues using spectroscopic
techniques. Protein Structure-function and
stability, drug-lipid and
protein-lipid interactions. Ecotoxicology. . Publications: more than 60 journal articles and
over 100 presentations. Recent
Selectes Publications: Cakmak G., Togan I., and Severcan F. “FT-IR
spectroscopic analysis of rainbow trout liver exposed to nonylphenol”
Applied Spectroscopy, 2003
(in press); Severcan F. and Haris P., “The main unfolding and aggregation of
PCS is preceded by the unfolding of a minor structure at lower temperature”
Biopolymers,
2003 inpress,; Toyran N.
and Severcan F., “Competitive
effect of vitamin D2 and Ca2+ on phospholipid model
membranes: An FTIR study” Chemistry and Physics of Lipids , inpress.; Boyar
H. and Severcan F., “ FTIR spectroscopic investigation of diabetic rat bone
tissues”, Spectroscopy,
2003 in press; Severcan
et al, “FTIR spectroscopic investigation of rat heart crude
membranes”.Spectroscopy, 2003, in press; Severcan
et al, “Tamoxifen destabilises model membranes at high concentrations” Bioscience Reports
, 20, 177-184, 2000; Toyran N.
and Severcan F., “Infrared
spectroscopic studies on the dipalmitoyl phosphatidylcholine bilayer
interactions with calcium phosphate: Effect of vitamin D2”, Spectroscopy,
16, 399-408, 2002.; Kazancı
N., Toyran N., Haris I. P., Severcan
F., “Vitamin D2 at high
and low concentrations exert opposing effects on molecular order and dynamics of
dipalmitoyl phosphatidylcholine membranes”, Spectroscopy, 15, 47-55, 2001. Severcan
M., Severcan, F. and Haris I.P, “Estimation
of protein secondary structure from FTIR spectra usıng neural networks”, J.
Molecular Structure, 565-566,
383-387, 2001. Severcan
F., Kazancı, N., and Zorlu, F., “Tamoxifen destabilises model
membranes at high concentrations” Bioscience
Reports , 20, 177-184, 2000.; Turan
B., Bayarı S., Balçık C., Severcan F.
and Akkaş N, “A biomechanical and spectroscopic study of bone from rats
with selenium deficiency and toxicity”, Biometals,
2000, 13, 113-121.; Severcan
F., Toyran N., Kaptan N., and Turan B., “FTIR study of the effect of diabetes
on rat liver and heart tissues in the C-H region", Talanta,
53, 55-59, 2000.
Inci
Togan (Prof.Dr): Gender: Female.
E-mail:
togan@metu.edu.tr. B.Sc.
Physics, METU, Turkey; M.A. Biophysics, The Johns Hopkins University, U.S.A.;
Ph.D. Biological Sciences, METU,Turkey. Schoolarships:
The Fulbright Scholarship for Res. Award in USA,1997;The Scholarship of ESEP
by Royal Society of England ,1988 International
Projects: “Concerted Action in Trout” EUproject (1998-2000). British
Council, Cardiff-ODTÜ joint project on genetic diversity of sheep, (2000-).
ECONOGENE, EU project (2001-). Research
Interests: Population genetics of
human, domestic sheep, trout, dogs; Biostatistics;
Ecotoxicology. Recent Publ.: Rainbow trout livers exposed to nonylphenol display
extensive structural and compositional changes on infrared spectroscopy
App. Spectro. in pr.;
Turkish population data on the CTTV STR loci
J. For. Sci.,in pr.; Alkylphenol concentrations in two rivers of Turkey
Env. Tox. and Phar.,in pr.;Developmental genetics and physiology of sex
differentiation in vertebrates (review)
Env. Tox., in pr.; DNA
diversity and population admixture in Anatolia
Am. J. of Phy. Anth.115: 144-,
2001.
Mete
SEVERCAN (Prof. Dr) : Gender: Male. Dept. of Electrical
and Electronic Engineering, METU. E-mail: severcan@metu.edu.tr.
B.S.
in Electrical Engineering, METU, Turkey; M.S. in Electrical Engineering, METU
Turkey; Ph.D. in Electrical Engineering, Stanford University, USA. Research
Associate in Stanford University, USA, Associate Professor in UC Berkeley, USA
and San Francisco State University, Research Associate in Perugia University,
Italy. Visiting Professor in BATTELLE, USA Schoolarships:
NATO, Fulbright; ICTP, Italy, British
Councli and Royal Society (U.K); Research Interests: Image Processing, Computer Vision,
Telecommunications,Bioinformatics. Recent
Projects: 1-“Protein Secondary Structure Determination from FTIR Data”
Royal Society Exchange Grant, 2001-2003 2-
Consultancy on Infrared Imaging
Systems and Image Processing, ASELSAN, 1996-2001. 3-
“Biophysical Studies of Protein Structure, Function and Stability (Protein
secondary structure prediction using neural networks)” British Council
Academic Link Project, 1997-2000.. Recent
Publ.: M. Severcan, F. Severcan, P. Haris, “Estimation of protein
secondary structure from FTIR spectra using neural networks”,
Journal of Molecular Structure, Vol. 565-566, pp383-387, May 2001.
Tülin Güray (Prof. Dr) : Gender: Female. Dept.of Biology, METU. E-mail: guray@metu.edu.tr
Research Interests: Purification,
characterization and kinetics of enzymes, free radicals, antioxidants and
polymorphism. Recent
Projects: 1- The investigation
of the relationship between the reactions of N-Acetylation and human breast. 2-
The study of relationship between N-Acetyltransferase and glutation S-transferase
teta polymorphism with breast cancer.. Selected
Publ.: T.Güray and T.Güvenç:”Characterization of sheep liver N-acetyltransferase”.
Biochem. Arch.,13, 107-111 , 1997. .T.Güray
and T.Güvenç:”Sheep tissue acetyl coenzyme A-dependent arylamine N-acetyltransferases.”Comp.Biochem. and Physiol., Vol.118C,No3,305-310, 1997.;
F.Severcan, Z.Yazıcı and T.Güray:”Interaction
of vitamin E-acetate with human erythrocyte membranes." Chem Phys Lipids, vol.101 (Editors F.Paltauf and H.H.O. Schmid)
Elsevier Publishing, Amesterdam. pp.176-177, 1999. Y.S. Geylan, S.Dizbay (Sak)
and T.Güray. “Arylamine N-acetyltransferase
activities in human breast cancer tissues"” Neoplasma,
48, No. 2, p.108-111, 2001.
Belma
Turan (Prof.Dr): Gender: Female. Dept. Biophysics, School of Medicine, Ankara Univ.
E-Mail: belma.turan@medicine.ankara.edu.tr Research interest: Effect of
antioxidants on the electophysiology of experimental diabetic cardiomyopathy:
Potential use of antioxidants on oxidant stress induced dysfunction of heart in
diabetes. Research Experience: 1- Cardiac
electrophysiology: Pathch-clamp, intracellular Ca2+ signalling, Ca2+-
and K+- ionic channels, 2-
Diabetes- and Oxidant- induced alterations in heart function:
Electrophysiological investigations.
3- Antioxidant effect on altered
heart functions. Publications: More than 50 journal articles and over 100
presentations. Selected publications: Ayaz
M., et al. Protective effect of selenium treatment on diabetes-induced
myocardial structural alterations. Biol.
Trace Elem. Res., 2002; 89(3): 215-226. Sayar K., et al. Effect of
dietary selenium intake on
-adrenergic
response of L-type Ca-current and
-adrenoceptor-adenylate
cyclase coupling in rat heart. J.
Nutrition, 2000, 130; 733-740.
Faruk
Zorlu (Prof. Dr): Gender:
Male. Dept. of Radiation Oncology, Medical School. Hacettepe University,Ankara.
Turkey. E-mail: farukzorlu@hotmail.com. M.D. Ankara Univ, Turkey; Ph.D.
Hacettepe University. Research Interests:
Radiation Oncology;
Selected Publication:
Malignancy in Behçet’s Disease: Report of thirteen cases and a review of the
literature, Clinical Rheumatology,
11: 875-880, 2001; Tamoxifen destabilises model membranes at high
concentrations, Bioscience Reports , 20, 177-184, 2000;Accelerated
hyperfractionated radiotherapy for supratentorial malignant gliomas, Radiotherapy
and Oncology 56, 233-238, 2000;Radiotherapy in the management of pituitory
adenomas, Radiotherapy and Oncology,
52: 233-237, 1999; Basal cell carcinoma developing in a case of medulloblastoma
associated with Gorlin’s Syndrome, Pediatric
Hematology and Oncology, 5(2): 187-191, 1998; Radiotherapy in the management
of agressive fibromatosis, The British
Journal of Radiology, 62: 854-856, 1989.
Tijen UTKAN (Prof. Dr):
Gender: Female, Address: Kocaeli
University Medical Faculty, Department of Pharmacology, Sopali, 41900
Derince-Kocaeli/TURKEY, Tel: +90 262 233 59 80 / 1187; Fax: +90 262 233 54 87;
E-mail: tijenutkan@hotmail.com
Research Interest: Diabetes mellitus, gastrointestinal pharmacology,
impotence. Recent Publications: 1- Utkan et al. Blood pressure and
vascular reactivity to endothelin-1, phenylephrine, serotonin, KCl and acetylcholine following chronic alcohol consumption in vitro.
Fundamental and Clinical Pharmacology, 15: 157-165, 2001. 2- Utkan et al.,
Chronic ethanol consumption impairs adrenoceptor-and
purinoceptor-mediated relaxations of isolated rat detrusor smooth muscle. British Journal of International, 88: 278-283, 2001.3- Utkan. et
al., Effects of the spesific phosphodiesterase inhibitors
on alloxan induced diabetic rabbit cavernous tissue in vitro. Int
J Impot Res.2001,13(1):24-30.
4. Utkan Et al. Impaired conraction and
relaxation in the aorta of streptozotocin-diabetic rats.
Pharmacology.
1998, 56(4):207-215
H. Kübra Paskaloglu (Dr.) Gender: female Marmara University Faculty of
Pharmacy, Department of Pharmacology Haydarpasa, ISTANBUL, TURKEY, Mobile Phone
: 0532 364 31 83; E-mail: kpaskaloglu@ixir.com
B.S.: M.Ü. Faculty of Pharmacy; M. Sc.and Ph.D degree, M.Ü. Health Sciences
Enstitute, Dept. Of Pharmacology, Ph.D., M.Ü.
Health Sciences Enstitute, Dept. Of Pharmacology. Ph.D thesis titled:
Investigation of the effects of melatonin treatment on streptozotocin induced
diabetic rat smooth muscles
and cavernous tissues in vitro" Research Interest: Farmakokinetics,
Diabetes Mellitus and treatment,Gastrointestinal diseases. SCIENTIFIC
PUBLICATION: 1. Protective Effect of Famotidine, Omeprazole and Melatonin
Against Acetylsalicylic Acid-Induced Gastric Damage in Rats, Digestive
Diseases and Sciences, 46(2): 318-330,2001. 2. Melatonin Protects Against
Gentamicin-induced Nephrotoxicity In Rats, Journal
of Pineal Research, 32:231-236,2002. 3. The Effects of Melatonin on Ischemia-Reperfusion
Induced Changes In Rat Corpus Cavernosum. The
Journal of Urology,167:2624-2627,2002. 4. Comparison of the Protective
Effects of Lansoprazole and Omeprazole Against AcetylSalicylic Acid-Induced
Gastric Damage In Rats. FABAD Journal of
Pharmaceutical Sciences, 27:77-84,2002.
Füruzan
YILDIZ (Assist.Prof.) Gender:
Female Kocaeli University Medical Faculty Department of
Pharmacology, Sopali, 41900 Derince-Kocaeli/TURKEY, mail: firuzanyildiz@superonline.com
B.S., M.S. PhD in Pharmacology Hacettepe University Faculty of Pharmacy; Research
Interest: Diabetes mellitus, gastrointestinal pharmacology, Publications: 1-
Impaired esophageal reactivity in adriamycin-inducedratesophageal atresia: An in
vitro study. Journal of Pediatric Surgery,36(10)October:
1569-1573, 2001.2- Blood pressure and vascular reactivity to endothelin-1,
phenylephrine, serotonin, KCl and acetylcholine following chronic alcohol
consumption in vitro.Fundamental and Clinical Pharmacology, 15: 157-165, 2001.3- Chronic
ethanol consumption impairs adrenoceptor-and purinoceptor-mediated relaxations
of isolated rat detrusor smooth muscle. British
Journal of International, 88: 278-283, 2001.
Nadide Kazancı (Prof. Dr.): Gender:
Female. Dept. of Physics, Ege University, Izmir. Turkey. E-mail: kazanci@sci.ege.edu.tr.
B.Sc. Physics, Ege Univ, Turkey; M.S. Physics, Ege Univ, Turkey; Ph.D. Physics,
Ege Univ, Turkey; Research Interests: Biophysical
studies of biological membranes and tissues.
Selected Publications: Vitamin D2 at high and low concentrations exert opposing
effects on molecular order and dynamics of dipalmitoyl phosphatidylcholine
membranes, Spectroscopy, 15:
47-55, 2001; Tamoxifen increases membrane fluidity at high concentrations, Bioscience
Reports, 20 (3): 177-184, 2000; Some peculiarities of nematic-isotropic
liquid phase transitions in monomorphic and ploymorphic mesogens, Material
Research Bull. 1892: 1-15, 2002 .
Şenay Hamarat Baysal
(Assistant Prof): Gender: Female. Department of Biochemistry. Ege University, Izmir, Turkey. B.Sc.
Chemistry-Biochemistry, Ege Univ, Turkey; M.S. Chemistry-Biochemistry, Ege
Univ., Turkey; Ph.D. Biochemistry, Ege Univ.,Turkey. Research Interests: Biochemistry of Enzymes; Antioxidant enzymes. Selected
Publication: In vitro study of
urease/AlaDH enzyme system encapsulated ınto human Erytrocytes and research
into its medical applications, Artif Cells Blood Subst. Imm. Biotech, 30:3,
2002; Encapsulation of PEG-urease/PEG-AlaDH Enzyme systems in erythrocytes,
Artif Cells Blood Subst. Imm. Biotech, 28:3, 2001; Immobilization of urease on
activated PEG, Artif Cells Blood Subst. Imm. Biotech, 24, 3: 273-278, 1996.
Sevgi Bayarı (Prof.Dr.)
Gender: Female. Addres:
Hacettepe University, Faculty of Education. Dept. of Physics. 06532, Beytepe,
Ankara.E-Mail:Bayari@hacettepe.edu.tr:
Research Interest: application of FTIR spectroscopy to biological tissues.
Computer simulation of small molecules. Selected
publications:İde, S.
Topaclı, A. and Bayarı,. S 1996
The Crystal and Molecular Structure of an Antidepressant Drug (Isocarboxazid)
, Crystal Res. Technol. 31(2) ,
p.187-195; Severcan F.and Bayarı S.and Karahan, D. 1999 FTIR and
turbitity study of fish oil-dipalmitoyl phosphatidylcholine model membrane
interactions, J.Mol.Struct. 480-481,
413-416Turan, B., Bayarı,S. Balcık,C. Severcan,F. and Akkas, N. 2000, A biomechenical and spectroscopic study of bone from
rats with selenium deficieny and toxicity, Biometals,
13(2), 113-121Bayarı S,
Ataç A. and Yurdakul Ş.
(2003), Coordination behaviour of nicotinamide: an
infrared spectroscopic study, J.Molecular Structure (accepted)
Kıvanc
Ergen (Assist. Prof.). Gender: Male. Kocaeli University Medical Faculty,Biophysics DepartmentSopali,
41900 Derince-Kocaeli/TURKEY,Tel.: +90 262 233 59 80-1700; Fax: +90 262 233 54 61 E-Mail:
akergen@superonline.com
Medical
Doctor-1992, PhD in Biophysics-1999, Research Interest ( Medical Biophysics,
Protein Synthesis Thesis Title: Stuructural
Studies on Active Sites of Elongation factor
Neslihan Toyran
Al-Otaibi: Gender:
Female, E-mail: toyran@metu.edu.tr
Education: B.Sc. (1997) and M.Sc. (1997-99) Dept. of Biology, Middle East
Technical University Ankara, Turkey Ph.D. Student since 1999 in Faculty of
Science, Dept. of Biology, Middle East Technical University Ankara, Turkey.
(Expected date of graduation: May 2003). Title: Fourier Transform
Infrared spectroscopic investigation of different pathological systems (Diabetes
mellitus and hypoperfusion) with various treatment methods. Academic
Work and Experience: Visiting researcher in Biophysical Structural Analysis
Laboratory, at Robert-Koch Institute, Berlin-Germany for a period of 6 months
with a scholarship from TÜBİTAK. RecentPublications: 1- Toyran, N. and Severcan F.,
“Competitive Effect of Vitamin D2 and Ca2+ on
Phospholipid Model Membranes: An FTIR Study”, 2002, In press in Chemistry and
Physics of Lipids. 2-
Toyran N. and Severcan F.,
“Infrared spectroscopic studies on the dipalmitoyl phosphatidylcholine
bilayer interactions with calcium phosphate: Effect of vitamin D2”,
Spectroscopy, 16, 399-408, 2002., 3. Kazancı N., Toyran N., Haris I. P., Severcan F., “Vitamin
D2 at high and low concentrations exert opposing effects on molecular
order and dynamics of dipalmitoyl phosphatidylcholine membranes”, Spectroscopy, 15, 47-55, 2001, 4.
Severcan,
F., Toyran, N., Kaptan, N. and Turan, B., Fourier Transform “Infrared Study of
the Effect of Diabetes on Rat Heart and Liver Tissues”, Talanta, 53: 55-59 (2000).
Handan BOYAR: Gender: Female, Address:
METU,
Dept. of Biology İnönü Bulv. 06531 Ankara /Turkey. E-mail:
boyarh@yahoo.com Education: B.Sc.
(1992) Middle East Technical University, Department of Biology, Ankara, Turkey.
M.Sc. (1995) Middle East Technical University, Department of Biology, Ankara,
Turkey Title of the Thesis: “Interaction
of estrogen and tamoxifen with phosholipid membranes: An FTIR study” Ph
D.: October 1998- Expected date of graduation: June 2003. Middle East Techn. Univ.,
Dept. of Biology, Ankara “Biophysical investigation of
the effects of antioxidants on normal and diabetic rat bone tissues at molecular
level.” Recent Publications
1- Boyar,H. Turan,B
Severcan, F. “FTIR spectroscopic
investigation of mineral structure in streptozotocin induced diabetic rat femur
and tibia, Spectroscopy, 2003, in press. 2- Boyar, H.,
Severcan,
F. “
Tamoxifen-Model Memmbrane Interactions: an
FTIR Study” Journal of Molecular Structure, 1997, 408/409:
265-268. 3- Boyar, H., Severcan, F.
˝Oestrogen-Phosholipid
Membrane Interactions: An FTIR Study˝ Journal of Molecular Structure, 1997, 408/409,
269-272.
Gülgün
ÇAKMAK,
Gender:
Female, Address: Middle East Technical University, Faculty of Science, Department of
Biology, 06531, Ankara, TURKEY. e-mail:
gulguncakmak@yahoo.com Education:
B.S. : 1997, Hacettepe
University, Faculty of Science, Departmet of Biology, Anakara, Turkey. M.S.
: June 2001, Middle East Technical University, Faculty of Science,
Department of Biology. Title of the Thesis: “
Spectroscopic Analysis of the Livers in Rainbow Trout (Oncorhynchus mykiss)
Exposed to Nonylphenol” Recent
Publications: 1) Çakmak, G., Uğuz,
C., Togan, İ. and Severcan, F. “FT-IR
Spectroscopic Analysis of Rainbow
Trout Liver Exposed to Nonylphenol” Applied
Spectroscopy, 2003, in press. 2)Çakmak,
G., Uğuz, C., Togan, İ. and
Severcan, F. “FT-IR Spectroscopic
Analysis of the Livers in Rainbow Trout (Oncorhynchus
mykiss) Exposed to Nonylphenol” XIIIth
National Biophysics Congress,
5-7 September 2001, Eskişehir, Turkey. (Oral Presentation)
Sevgi
TÜRKER, Gender:
Female,
E-mail:
sturker@metu.edu.tr
Address: Middle East Technical University, Department of Biology, 06531,
Ankara, TURKEY. Education: B.Sc.
(1998) Faculty of Science, Hacettepe University,
Turkey; MSc. (1999-2002)
Biophysics, METU, Turkey; Ph.D. Student since 2002 in Faculty of Science,
Department of Biology, METU, Ankara, Turkey. (Expected date of graduation:
2006). Publications : (2002) Poster Presentation in International Congress
of Biophysics, Research Subject: Applications of FT-IR Spectroscopy on
Different Biological Systems.
Güvenç GÖRGÜLÜ: Gender: Male B.Sc. (1994)
Faculty of Education, Marmara University, Turkey;
MSc. (1995-1997) Biochemistry Süleyman Demirel Uni., Turkey; Ph.D
Student since 1998 in Department of Biochemistry
METU, Türkiye, (Expected
date of graduation: September 2003). Title: The Effects of Antioxidants on the
Structure and Function of Some Rat Tissues and Membranes. Research
Interests: Antioxidants, diabetes Academic
Work and Experience: (1997) Participation
Enzymology Summer School, Türkiye; (2002)
Participation Proteomics
Summer School, Turkey. Publications: (1997)
Poster Presentation in the 1st Congress Of Free Radicals &
Antioxidants, Turkey; (1997) Poster Presentation in Gevher Nesibe Medical Days;
(2002) Poster Presentation in International Congress of Biophysics Microsomal
Lipid Peroxidation.
Ayca
Dogan: Gender:
Female,
E-mail:
aycci@yahoo.com
Adress: Kocaeli University Hereke
Omer Ismet Uzunyol MYO Marshall Campus, Kocaeli, TURKEY. I. Education: : B.Sc.
(1996) Department of Biology, Gazi University, Ankara, Turkey; M.Sc. (1998-2001)
Department of Biotechnology, METU Ankara, Turkey. Academic
Work and Experience: Instructor
since 2002 in Kocaeli University University Hereke Omer Ismet Uzunyol
MYO Marshall Campus, Kocaeli, Turkey. Internship on MonoclonalAntibodies and
Elisa techniques in the Department of Biotechnology of Turkish Atomic Energy
Authority (1996). Project on Chromosome Bands in Gazi University (1995). Publications:
1- A.Dogan ,“The Effects of Gamma
Irradiation on Hazelnut Tissue at Molecular Level Fourier Transform Infrared
Spectroscopy”, MS. Thesis 2001
Evren KOBAN, Gender: Female -mail: evrenkoban@yahoo.com
Adress: Middle East Technical
University, Department of Biology, 06531, Ankara, TURKEY.
Education: Ph.D. Student since
2001 in Faculty of Science, Department of Biology, Middle East Technical
University Ankara, Turkey. Research Int.:
Population genetics, biodiversity and conservation. Projects participated: VHAG 1396 (on brown trout), TOGTAG/TARP-1811
(on rainbow trout), VHAG-1553
and TBAG-2127 (both on sheep) which are all supported by the Turkish Scientific
and Technical Research Council; British Council, Cardiff-ODTÜ joint project on
genetic diversity of sheep, (2000-); ECONOGENE, EU project (2001-). Experience: Research scholar in Veterinary Genetics Laboratory (VGL)
in 2000, UC Davis, California, USA to study population genetics and diversity in
Turkish sheep dogs. Visiting scholar (from the British Council Turkey) in School
of Biosciences, Cardiff University, UK to study population genetics and
biodiversity of Anatolian sheep.
Publications
[METU1]
Cakmak G., Togan I., and Severcan F. “FT-IR spectroscopic analysis of
rainbow trout liver exposed to nonylphenol”
Applied Spectroscopy, 2003
(in press);
[METU2]
Severcan F. and Haris P., “The main unfolding and aggregation of
PCS is preceded by the unfolding of a minor structure at lower temperature”
Biopolymers,
2003 inpress,;
[METU3]
Toyran
N. and Severcan F., “Competitive
effect of vitamin D2 and Ca2+ on phospholipid model
membranes: An FTIR study” Chemistry and
Physics of Lipids , inpress.;
[METU4]
Boyar,H. Turan,B Severcan, F.
“FTIR spectroscopic investigation of mineral structure in streptozotocin
induced diabetic rat femur and tibia, Spectroscopy, 2003, in
press.
[METU5]
Severcan F., Kaptan N., Turan B.,“FTIR spectroscopic investigation of rat
heart crude membranes”.Spectroscopy,
2003, in press;
[METU6]
Severcan F., Kazanci N., Zorlu F., “Tamoxifen destabilises model
membranes at high concentrations” Bioscience
Reports , 20, 177-184, 2000;
[METU7]
Toyran
N. and Severcan F., “Infrared
spectroscopic studies on the dipalmitoyl phosphatidylcholine bilayer
interactions with calcium phosphate: Effect of vitamin D2”, Spectroscopy, 16, 399-408, 2002.;
[METU8]
Kazancı
N., Toyran N., Haris I. P., Severcan
F., “Vitamin D2 at high
and low concentrations exert opposing effects on molecular order and dynamics of
dipalmitoyl phosphatidylcholine membranes”, Spectroscopy, 15, 47-55, 2001.
[METU9]
Severcan
M., Severcan, F.
and Haris I.P, “Estimation of protein secondary structure from FTIR spectra usıng
neural networks”, J. Molecular
Structure, 565-566, 383-387,
2001.
[METU10]
Severcan
F., Kazancı, N., and Zorlu, F.,
“Tamoxifen destabilises model membranes at high concentrations” Bioscience Reports , 20, 177-184, 2000.;
[METU11]
Turan
B., Bayarı S., Balçık C., Severcan F.
and Akkaş N, “A biomechanical and spectroscopic study of bone from rats
with selenium deficiency and toxicity”, Biometals,
2000, 13, 113-121.;
[METU12]
Severcan
F., Toyran N., Kaptan N., and Turan B., “FTIR study of the effect of diabetes
on rat liver and heart tissues in the C-H region", Talanta,
53, 55-59, 2000.
[METU13]
Severcan. F, Durmuş. O.H., Eker F., P. Haris, Akınıoğlu B.G.
“Vitamin D2 modulated melittin--membrane interactions”, Talanta
53, 205-211, 2000.
[METU14]
Toyran N., and Severcan
F., “The effect of magnesium ions on vitamin D2-phospholipid
model membrane interactions in the presence of different buffer media”
Talanta, 53, 23-27, 2000.
[METU15] Severcan
F, Bayarı S, Karahan D., “An FTIR and turbidity studies of fish oil-dipalmitoylphosphatidtlcholine
model membrane interactions”, J.
Molecular Structure, 480-481, 413-416, 1999.
[METU16]
Eker F, Durmuş H.O, Akınoğlu B.G. and Severcan
F., Application of turbidity technique on peptide-lipid and drug-lipid
interactions, J. Molecular Structure,
482-483, 693-697, 1999.
[METU17]
Haris P and Severcan F, FTIR
spectroscopic characterization of protein structure in aqueous and non-aqueous
media, J. Molecular Catalysis: B
(Enzymatic), 7, 207-221 (1999).
[METU18] T.Güray and T.Güvenç:”Characterization of sheep
liver N-acetyltransferase”. Biochem.
Arch.,13, 107-111 , 1997.
[METU19]
T.Güray and T.Güvenç:”Sheep tissue acetyl coenzyme A-dependent arylamine N-acetyltransferases.”Comp.Biochem.
and Physiol., Vol.118C,No3,305-310, 1997.;
[METU20]
F.Severcan, Z.Yazıcı and T.Güray:”Interaction of vitamin E-acetate
with human erythrocyte membranes." Chem
Phys Lipids, vol.101 (Editors F.Paltauf and H.H.O. Schmid) Elsevier
Publishing, Amesterdam. pp.176-177, 1999.
[METU21]
Y.S. Geylan, S.Dizbay (Sak) and T.Güray.
“Arylamine N-acetyltransferase activities in human breast cancer
tissues"” Neoplasma, 48, No.
2, p.108-111, 2001.
[METU22]
Ayaz M., et al. Protective effect of selenium treatment on
diabetes-induced myocardial structural alterations. Biol. Trace Elem. Res., 2002;
89(3): 215-226.
[METU23]
Sayar K., et al. Effect of dietary selenium intake on
-adrenergic response of
L-type Ca-current and
-adrenoceptor-adenylate
cyclase coupling in rat heart. J.
Nutrition, 2000, 130; 733-740.
[METU24]
Çetinkaya, G., Ülküer, K.Ü., Togan, İ., (2003). Turkish population dta
on the CTTV STR loci. Journal of Forensic Seciences (in press).
[METU25]
Benedetto, G.D., Ergüven, A., Stenico, M., Castri, L., Bertorelle, G., Togan,
İ., Barbujani, G., (2001). DNA diversity and population admixture in
Anatolia. American Journal of Physical Anthropology, 115: 144-156.
[METU26]
Altunok V., Maden, M., Nizamlıoğlu, M. and Togan, İ. (2001). Some
of the frequently used biochemical values of serum and plasma in three different
populations of Anatolian shepherd dog. Revue Méd. Vét. 152 (3):261-264
[METU27]
Altunok, V., Nizamlıoğlu, M., Ergüven, A., Togan, İ., 1999. Red
blood cell enzyme biochemical polymorphism in Anatolian sheperd dog, Revue
Méd. Vét., 150,7, 625-628.
[METU28]
Zorlu et al., Malignancy in Behçet’s Disease: Report of thirteen cases and a
review of the literature, Clinical
Rheumatology, 11: 875-880, 2001;
[METU29]
Zorlu et al., Accelerated hyperfractionated radiotherapy for supratentorial
malignant gliomas, Radiotherapy and
Oncology 56, 233-238, 2000;
[METU30]
Zorlu et al. Radiotherapy in
the management of pituitory adenomas,
Radiotherapy and Oncology, 52: 233-237, 1999;
[METU31]
Zorlu et al Basal cell carcinoma developing in a case of medulloblastoma
associated with Gorlin’s Syndrome, Pediatric
Hematology and Oncology, 5(2): 187-191, 1998;
[METU32]
Zorlu et al Radiotherapy in the management of agressive fibromatosis, The
British Journal of Radiology, 62: 854-856, 1989.
[METU33]
Utkan.
et al., Effects of the spesific phosphodiesterase inhibitors on alloxan induced
diabetic rabbit cavernous tissue in vitro. Int
J Impot Res.2001,13(1):24-30.
[METU34]
Utkan Et al. Impaired
conraction and relaxation in the aorta of streptozotocin-diabetic
rats. Pharmacology. 1998, 56(4):207-215
[METU35]
Hamarat
et al., Blood pressure and vascular reactivity to endothelin-1, phenylephrine,
serotonin, KCl and acetylcholine following chronic alcohol consumption in vitro.Fundamental
and Clinical Pharmacology, 15: 157-165, 2001.
[METU36]
Hamarat et al.
In vitro study of urease/AlaDH enzyme system encapsulated ınto human
Erytrocytes and research into its medical applications, Artif Cells Blood Subst.
Imm. Biotech, 30:3, 2002;
[METU37]
Hamatrat et al Encapsulation of PEG-urease/PEG-AlaDH Enzyme systems in erythrocytes,
Artif Cells Blood Subst. Imm. Biotech, 28:3, 2001;
[METU38]
Hamarat et al Immobilization of urease on activated PEG, Artif Cells Blood
Subst. Imm. Biotech, 24, 3: 273-278, 1996.
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